Table 3.
AP properties after laminectomy and SCI following PKC activation
| All neurons | Laminectomy + PMA | SCI + PMA | |||||
|---|---|---|---|---|---|---|---|
| Naïve | LAM | SCI | Pre | Post | Pre | Post | |
| RMP (mV) | -56.9 ± 2.1 | -57.9 ± 2.3 | -56.0 ± 1.1 | -56.6 ± 3.3 | -68.1 ± 3.3** | -56.2 ± 2.5 | -62.3 ± 2.3** |
| Input Res. (GΩ) | 0.6 ± 0.1 | 0.7 ± 0.1 | 0.6 ± 0.1 | 0.7 ± 0.2 | 1.0 ± 0.2* | 0.6 ± 0.2 | 0.6 ± 0.2 |
| Capacitance (pF) | 15.5 ± 1.3 | 17.5 ± 1.0 | 21.8 ± 1.4* | ||||
| Diameter (μm) | 19.5 ± 0.6 | 22.2 ± 0.5 | 22.4 ± 0.4 | ||||
| Rheobase (pA) | 73 ± 23 | 108 ± 26 | 101 ± 12 | 53 ± 15 | 89 ± 29 | 83 ± 21 | 140 ± 34** |
| Threshold (mV) | -32.5 ± 1.1 | -31.1 ± 1.5 | -31.0 ± 1.0 | -31.0 ± 2.8 | -37.1 ± 2.5* | -30.7 ± 1.4 | -33.5 ± 1.5 |
| Overshoot (mV) | 30.7 ± 4.2 | 28.3 ± 3.4 | 26.9 ± 2.0 | 40.7 ± 3.0 | 31.7 ± 2.5** | 32.9 ± 4.1 | 22.2 ± 3.9** |
| AHP (mV) | -64.5 ± 1.3 | -69.6 ± 2.1 | -68.5 ± 1.0 | -71.9 ± 1.8 | -80.2 ± 2.5** | -70.6 ± 1.3 | -76.5 ± 2.4* |
| APD50 (ms) | 6.4 ± 1.1 | 6.8 ± 0.9 | 5.9 ± 0.5 | 5.1 ± 0.4 | 4.2 ± 0.3** | 5.2 ± 0.5 | 4.7 ± 0.4 |
| APD90 (ms) | 1.56 ± 0.26 | 1.66 ± 0.20 | 1.52 ± 0.09 | 1.18 ± 0.11 | 1.04 ± 0.07* | 1.40 ± 0.09 | 1.41 ± 0.13 |
| Max Dep. rate (mV/ms) | 46.9 ± 8.7 | 44.8 ± 7.4 | 40.0 ± 3.9 | 56.5 ± 11.9 | 66.0 ± 9.4 | 43.3 ± 4.9 | 46.6 ± 7.8 |
| Max Rep. rate (mV/ms) | 26.1 ± 4.1 | 25.1 ± 3.1 | 25.1 ± 2.1 | 33.4 ± 3.5 | 33.9 ± 3.5 | 26.0 ± 2.8 | 23.3 ± 3.2 |
| N | 13 | 21 | 43 | 8 | 11 | ||
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AP properties were measured in naïve, laminectomy (Lam), and SCI neurons as described in Materials and Methods, and ANOVA used for comparisons. For PMA wash in experiments, paired t tests were used for comparisons;
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↵* p ≤ 0.05;
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↵** p ≤ 0.01. RMP, resting membrane potential; Dep., depolarization; Rep., repolarization.